PrepGEM is formulated for general DNA extraction from buccal swabs and saliva. PrepGEM Saliva is a preparative method for DNA extraction. The prepGEM method lyses cells and removes nucleoproteins from the DNA. Extracted DNA can be used for many types of genotyping including SNP and STR analysis as well as quantitative, multiplex and end-point PCR.ZyGEM’s prepGEM™ DNA extraction technology, based on the proprietary EA1 enzyme, can extract high quality DNA from a broad range of sample materials in less than 20 minutes.
The extracted DNA can be used directly with analytical platforms based on PCR, quantitative PCR or capillary electrophoresis. Unlike most DNA preparation methods that require multiple steps and special handling, which increase the risk of error or contamination, ZyGEM’s DNA extraction technology uses a single tube and a simple two-step temperature cycle to prepare DNA for analysis, greatly reducing sample preparation time and the risk of sample contamination.
All prepGEM™ kits are formulated and QCed for animal testing, biosecurity and R&D applications.
prepGEM™ Saliva is a single tube, enzymatic DNA extraction system specifically optimised for the extraction of DNA from buccal swabs and saliva. The kit uses sequential enzymatic treatment to lyse the cells and prepare the DNA template in conjunction with a specific wash and extraction buffer.