prepGEM™ DNA Extraction – Blood

prepGEM™ Blood is a simple enzymatic DNA extraction system specifically optimised to overcome the PCR inhibition commonly found with single tube extraction protocols for blood. It is ideal for high throughput genotyping applications, PCR applications, or SNP detection: whenever rapid results are required.

 

The availability of simple DNA sample preparation methods is becoming increasingly important in fast-paced research settings, as the use of nucleic acid-based analyses continues to expand rapidly. ZyGEM’s prepGEM™ DNA extraction technology, based on the proprietary EA1 enzyme, can extract high quality DNA from a broad range of sample materials in less than 20 minutes.

 

The extracted DNA can be used directly with analytical platforms based on PCR, quantitative PCR or capillary electrophoresis. Unlike most DNA preparation methods that require multiple steps and special handling, which increase the risk of error or contamination, ZyGEM’s DNA extraction technology uses a single tube and a simple two-step temperature cycle to prepare DNA for analysis, greatly reducing sample preparation time and the risk of sample contamination.

 

All prepGEM™ kits are formulated and QCed for animal testing, biosecurity and R&D applications.

  • The kit uses optimized methods and formulations to provide DNA in 30 minutes

 

  • There are no multiple centrifugal steps, washes, vacuums, or columns

 

  • Reactions are closed-tube and hands-off

 

  • All buffers and reagents are PCR compatible, so no downstream purification is required

 

  • Closed–tube (protects sample integrity) Hands–off (saves labor costs)

 

  • One-step (maximizes yields and so decreases repeat rate)

 

  • Easily automated (compatible with most low cost, off-the-shelf liquid handling robots)
  • Forensics- Biology & DNA

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